ZFIN ID: ZDB-FIG-191230-1703
Soman et al., 2019 - Restriction of mitochondrial calcium overload by mcu inactivation renders neuroprotective effect in Zebrafish models of Parkinson's disease. Biology Open   8(10) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 5.

Mitochondrial dynamics is alteredin pink1−/− zebrafish. (A–L) Representative images of immunohistochemistry performed on wt, pink1−/−, mcu−/− and (pink1; mcu)−/− dissected 4 dpf zebrafish larval brain. DA neurons (red) are marked with anti-TH antibody, mitochondrial structures (green) are marked with anti-Tom20 antibody and DAPI (blue) is shown as nuclear stain. Arrowheads show mitochondrial morphology in muscle fibers. (M–O) Representative images of mitochondrial volumetric analysis in muscle fibers of transgenic wt, pink1−/− and (pink1; mcu)−/− 3 dpf zebrafish expressing mitochondria-localized GFP. Arrowheads point out mitochondrial structures in somites. (P) Graphical representation and statistical analysis of mitochondrial area with one-way ANOVA and post-hoc analysis using Tukey’s multiple comparison test of two different experiments with n=15. There was a significant (*P>0.05) decrease in DA neuronal mitochondrial area in pink1−/− zebrafish when compared to wt; mitochondria area was restored (**P>0.01) in (pink1; mcu)−/− zebrafish. (Q,R) Graphical representation and statistical analyses of muscle mitochondrial volume and sphericity with one-way ANOVA and post-hoc analysis using Tukey’s multiple comparison test of three different experiments with n=30. There was a slight decrease in mitochondrial volume in pink1−/− zebrafish when compared to wt. The cumulative sphericity index is significantly (***P>0.001) reduced in pink1−/− zebrafish, unlike wt and (pink1; mcu)−/− zebrafish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP cms1Tg (AB) standard conditions Protruding-mouth skeletal muscle cell mitochondrion IFL
pink1sh397/sh397; cms1Tg (AB) standard conditions Protruding-mouth skeletal muscle cell mitochondrion IFL
pink1sh397/sh397; mcush214/sh214; cms1Tg (AB) standard conditions Protruding-mouth skeletal muscle cell mitochondrion IFL
th Ab3-th AB standard conditions Day 4 dopaminergic neuron IHC
Ab3-th pink1sh397/sh397 (AB) standard conditions Day 4 dopaminergic neuron IHC
Ab3-th pink1sh397/sh397; mcush214/sh214 (AB) standard conditions Day 4 dopaminergic neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-tomm20 IHC AB standard conditions Day 4 dopaminergic neuron mitochondrion
IHC pink1sh397/sh397 (AB) standard conditions Day 4 dopaminergic neuron mitochondrion
IHC pink1sh397/sh397; mcush214/sh214 (AB) standard conditions Day 4 dopaminergic neuron mitochondrion
Phenotype Details
Fish Conditions Stage Phenotype
pink1sh397/sh397 (AB) standard conditions Day 4 dopaminergic neuron mitochondrion decreased area, abnormal
pink1sh397/sh397; cms1Tg (AB) standard conditions Protruding-mouth skeletal muscle cell mitochondrion decreased volume, abnormal
Protruding-mouth skeletal muscle cell mitochondrion deformed, abnormal
pink1sh397/sh397; mcush214/sh214 (AB) standard conditions Day 4 dopaminergic neuron mitochondrion area, ameliorated
pink1sh397/sh397; mcush214/sh214; cms1Tg (AB) standard conditions Protruding-mouth skeletal muscle cell mitochondrion decreased volume, abnormal
Protruding-mouth skeletal muscle cell mitochondrion morphology, ameliorated
Acknowledgments:
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