FIGURE

Fig. 4

ID

ZDB-FIG-200520-17

Publication

Soman et al., 2019 - Restriction of mitochondrial calcium overload by mcu inactivation renders neuroprotective effect in Zebrafish models of Parkinson's disease

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Fig. 4

Mitochondrial membrane potential partly restored after mcu deletion. The graph represents ratio of JC-1 dye emission spectrum (590?nm to 530?nm). wt mitochondria showed a slight uptake of JC-1, which turned into leakage after ?18?min after the start of the experiment, when mitochondria began to lose membrane integrity in an in vitro environment. pink1^−/− mitochondria did not show uptake, instead we observed JC-1 leakage starting at ?6?min after experiment initialization. Both variants started and ended at similar levels. Results are mean with s.e.m. [wt had two, pink1^−/− had two and pink1; mcu)^−/− had one independent replication(s)] gathered during two independent experiments. Every replication was done on 200 larvae per variant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pink1^sh397/sh397 (AB) pink1^sh397/sh397; mcu^sh214/sh214 (AB)
Observed In:	regulation of mitochondrial membrane potential
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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