Figure 2

Quantification of CD31⁺ cells in endometriotic lesions in the murine model. (A) Representative immunofluorescence sections of lesions from PBS‐ or rmIL‐10‐treated mice. Blue, nucleus (DAPI); red, CD31 (Alexa Fluor 568). Red scale bars = 20 µm. (B) Representative dot plots analysed by TissueQuest software. (C) The frequency of CD31⁺ cells among DAPI⁺ cells in the whole field of each lesion. IC, isotype control; αmIL‐10, mAb against mouse IL‐10. One dot represents one section from each endometriotic lesion. One endometriotic lesion was sampled per mouse. n = 4 mice in the PBS/rmIL‐10‐treated group; n = 5 mice in the IC/αmIL‐10‐treated group. (D, E) Representative immunohistochemistry sections of lesions for Ki‐67 or active caspase‐3 (brown colour) from αmIL‐10‐treated mice. Blue, haematoxylin counterstain. Black scale bar (left panels) = 200 µm; green scale bar (right panels) = 20 µm. (F, G) The frequency of Ki‐67⁺ cells or active caspase‐3⁺ cells among haematoxylin⁺ cells in the whole field of each lesion. One dot represents one section from each endometriotic lesion. Two endometriotic lesions per treatment were from one mouse. n = 3 mice in the IC/αmIL‐10‐treated group. The horizontal dashed line within the vertical points marks the mean for each group. The data represent one of at least two independent experiments with consistent results.