Figure 5

Analysis of CD123, CD31, IL‐10, and IL‐10R expression in human endometrioma and uterine endometrial tissues. (A) Immunohistochemistry for CD123, CD31, IL‐10, or IL‐10R (brown colour) in a representative endometrioma lesion as well as the fibrosis area and normal area surrounding the corresponding endometrioma lesion. The insets (solid box) show two‐fold enlarged images of vascular structure (V) or glandular epithelium (G) (dashed box) with the same colour. Arrows indicate CD123⁺ cells. Blue, haematoxylin counterstain. Black scale bars = 50 µm. The enlarged images of IL‐10 and IL‐10R expression on endometrioma lesions in A are shown in supplementary material, Figure S7 for clear presentation. (B) Quantification of positive cell frequencies in each endometrioma lesion and corresponding fibrosis area and/or normal tissue analysed by HistoQuest software. Number of endometrioma lesion samples: 11 for all markers; number of fibrosis areas within the endometrioma lesion samples: 11 for all markers; number of normal regions within the endometrioma lesion samples: 8 for CD123, 2 for CD31, 7 for IL‐10, and 5 for IL‐10R. p < 0.05 was considered significant based on the Kruskal–Wallis test and then Dunn's multiple comparison test. (C) Double immunohistochemical staining for IL‐10 (red) or/and CD123 (brown) in a representative human endometrioma lesion. NC, negative control with omission of both primary antibodies. The insets (solid box) show two‐fold enlarged images of positive cells as indicated (dashed box). Arrows indicate IL‐10⁺ CD123⁺ pDCs with voluminous cytoplasm and smooth cell surface. Blue, haematoxylin counterstain. Red scale bars = 20 µm.