Figure 3

Lymphatic differentiation defects in mir-204-deficient animals.

(a) Quantitative TaqMan RT-PCR measurement of the relative levels of mature mir-204 in one dpf mir-204–1-/- mutants and wildtype siblings, normalized to wild type levels. (b,c) Confocal images of 2 dpf Tg(mrc1a:eGFP)y251, Tg(kdrl:mCherry)y171 mir-204–1-/- mutant (c) or MO2-injected mir-204–1-/- mutant (d) animals. (d) Quantitation of the number of PL sprouts in animals as in panels b and c. Scale bar: 100 μm (b). All graphs are analyzed by t-test and the mean ± SD is shown.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
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