Figure 2—figure supplement 2.

The effects of single or combined injections of mir-204 targeting MOs.

(a) Sequence alignment of MO1, 2, 3. (b-h) Representative confocal images of 5 dpf Tg(mrc1a:eGFP)y251, Tg(kdrl:mCherry)y171 double-transgenic animals injected with 0.5 ng of MO1 (b), MO2 (c), or MO3 (d); injected pairwise with 0.5 ng each of MO1+MO2 (e), MO1+MO3 (f), or MO2+MO3 (g), for a final combined MO dose of 1.0 ng in each case; injected with 0.5 ng each of MO1+MO2+MO3 (h), for a final combined MO dose of 1.5 ng. (i) Quantitation of the single MO injections in panels b-d. (j) Quantitation of the combined MO injections in panels e-h. All images are lateral views, rostral to the left. Scale bar: 100 μm (b). All graphs are analyzed by t-test and the mean ± SD is shown.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
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