FIGURE 6

Mitochondrial network maturation is conditional to myoblast fusion. (A,B) Confocal imaging of mitochondrial network (Tomm20, T20; green) counterstained with Phalloidin (Ph; red) and Hoescht (H; blue) at 28 hpf (A) and 48 hpf (B); control (Ctrl, mock injection) or Myomaker-targeting Morpholinos (Mo Myomaker). (C) Electron micrographs of longitudinal sections at 48 hpf in control and Myomaker depleted embryos. Top images are close to boundaries, bottom images are at the somite center. (D) Quantification of mitochondrial number and area at 48 hpf from longitudinal electron micrographs (n = 6 micrographs for Ctrl, n = 7 micrographs for Mo). (E) Quantification of Tomm20-zsGreen fluorescence ratio between somite center and boundary region 48 hpf (n = 4 fish per group, 3 images analyzed per fish). (F) Cartoon representing somite structure and mitochondrial network at 48 hpf in control and Myomaker depletion. Bars are mean ± SEM. ^∗∗∗P < 0.001, unpaired T-test.