FIGURE 3

Electron transport chain supercomplexes are qualitatively stable throughout zebrafish development. (A–E) Representative BN-PAGE experiment with mitochondrial extracts from adult fish (6 months), 18, 24, 48 hpf, and 5 dpf. Specific antibodies against individual ETC complexes were used separately. Red numbers correspond to new bands appearing at particular time points. HMW is high molecular weight, int is intermediate. (F) Quantification of individual ETC complexes. Each value represents the signal on the immunoblot for each ETC complex (n = 2, each n with 250 embryos per group). (G) Quantification of supercomplexes (SCs). Each value represents the sum of all SCs bands including III₂ + IV, III₂ + IV₂, I + III₂, I + IV, I + III₂ + IV, I + III₂ + IV_n and HMW SCs (n = 2, each n with 250 embryos per group). (H–J) Quantification of the percentage of CI, CIII, and CIV in free form and in SCs (n = 2, each n with 250 embryos per condition). Bars are mean ± SEM. ^∗P < 0.05, ^∗∗∗P < 0.001, two way repeated measures ANOVA (line is effect of time) or one way repeated measures ANOVA with Tukey HSD post hoc comparison (bracket). See also Supplementary Figure S6.