FIGURE

Fig. 3

ID

ZDB-FIG-190925-21

Publication

Gath et al., 2019 - Zebrafish mab21l2 mutants possess severe defects in optic cup morphogenesis, lens and cornea development

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Fig. 3

mab21l2^−/− embryos display transient increase in cell death, decreased proliferation in the developing lens. A‐C,F‐H: TUNEL stain of wild‐type (A‐C) and mab21l2^−/− mutant (F‐H) embryos. Compared with wild‐type, mab21l2^−/− mutants possess a transient increase in cell death at 22SS (F) and 26SS (G), while 48 hpf (H) embryos have equivalent proportions of dying cells to their wild‐type siblings (B, A, and C, respectively). K‐M: Quantification of TUNEL⁺ cells in images A‐C,F‐H. Note significant differences in proportion of TUNEL⁺ cells in 22SS (K; P < 0.0001) and 26SS (L; P = 0.001) but not 48 hpf (M; P = 0.63) samples. D,E,I,J: BrdU incorporation assay in wild‐type (D,E) and mab21l2^−/− mutant (I,J) embryos. Compared with wild‐type (D), mab21l2^−/− (I) mutants possess a decrease in proliferative cells in the lens at 26SS. N,O: Quantification of BrdU⁺ cells in images D,E,I,J. Note significant differences in the proportion of BrdU⁺ lens cells in 26SS (N) samples. Dorsal is up in all panels. Scale bars = 50 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mab21l2^au10/au10 (AB)
Observed In:	lens cell death lens cell population proliferation
Stage Range:	20-25 somites to 26+ somites

Phenotype Detail

Acknowledgments

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