Figure 4—figure supplement 1.

(A) Dose response curve of the glucose lowering effect of flutamide in ins mutant larvae, treated from 84 to 120 hpf; mean ± SEM, n = 3 replicates. (B) Wholemount in situ hybridization for ar expression in 120 hpf wild-type and ins mutant larvae showing signal in the brain and liver (red arrowheads). (C) The 12 genes differentially regulated in ins mutants compared to non-mutant siblings and modulated in the opposite direction upon treatment with flutamide or cyproterone compared to DMSO; listed with their fold change (Log₂ scale) and expression levels under control condition (base mean reads); genes are ordered by the fold change in the flutamide vs DMSO condition. (D) Schematic of insig1 and btg2 gene loci, with location of transcription start site (TSS) and androgen response elements (ARE, blue arrowheads). (E) Schematic of vehicle vs flutamide treatment of Tg(ins:NTR) adult animals following β-cell ablation with metronidazole (MTZ) injection to induce hyperglycemia. Scale bar: 250 μm.