FIGURE

Figure 1—figure supplement 1.

ID

ZDB-FIG-190723-1912

Publication

Mullapudi et al., 2018 - Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists

Other Figures

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Figure 1—figure supplement 1.

<italic>ins,</italic> and not <italic>insb</italic>, is the predominant paralog expressed in zebrafish pancreatic islets.

(A) Brightfield images of 72 hpf wild-type, ins mutant and insb mutant larvae. (B–C) Confocal plane images of the pancreatic islet in 72 hpf wild-type and insb mutant larvae stained for Insulin (white). (D) Fasting blood glucose levels in three mpf wild-type and ins ± animals; mean ± SEM, n = 11 animals. (E) Body length measurements of 50 dpf wild-type and ins ± animals; mean ± SEM, n = 5–6 animals. (F) mRNA expression time course of ins and insb during zebrafish development from 0 to 120 hpf. (G) 4 ng of control (ctrl) or ins morpholino (MO) was injected in non-transgenic or Tg(ins:insb) (insb OE) embryos at the one cell stage and glucose levels measured at 96 hpf; mean ± SEM, n = 3 replicates. Scale bars: 250 μm (A), 5 μm (C, D). P values from t-tests.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	insb^{bns295/bns295}
Observed In:	whole organism whole organism glucose
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

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