FIGURE

Figure 1

ID

ZDB-FIG-190723-1911

Publication

Mullapudi et al., 2018 - Screening for insulin-independent pathways that modulate glucose homeostasis identifies androgen receptor antagonists

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

(A) CRISPR target site in the insulin gene, with PAM sequence highlighted, and the resulting 16 bp deletion allele (below). (A’) Schematic of wild-type Insulin protein and the predicted mutant protein which contains novel sequence (black). (B) Confocal projection images of the pancreatic islet in 96 hpf Tg(ins:GFP) ins +/+ and ins -/- animals immunostained for Insulin (red) and Glucagon (cyan). (C) Free glucose levels in wild-type and mutant animals from 1 to 6 dpf; mean ± SEM, n = 2–4 replicates. (D) Nile Red staining (green) for neutral lipids in 120 hpf wild-type (top) and mutant (bottom) larvae, with yolk lipid content outlined (yellow dots). (E) Genotype distribution from ins ± incross, calculated as the percentage of total animals at each stage; mean ± SEM, n = 32 animals at each stage. (F) Heat map of the proteomic signature of zebrafish ins mutants at 120 hpf compared to signatures from rodent (R) and human (H) diabetic proteome studies. Canonical pathways implicated in most studies are listed first. P-value cut-off set at <0.05. Scale bars: 10 μm (B), 500 μm (D).

Expression Data

Gene:	GFP
Antibody:	Ab1-ins
Fish:	zf5Tg ins^{bns102/bns102}; zf5Tg
Anatomical Term:	endocrine pancreas
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ins^{bns102/bns102} ins^{bns102/bns102}; zf5Tg
Observed In:	endocrine pancreas whole organism whole organism glucose yolk lipid
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife