ZFIN ID: ZDB-FIG-190723-158
Hartwell et al., 2019 - Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression. PLoS Genetics   15:e1008051 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 26+ somites to Prim-25
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 26+ somites to Prim-25

Fig 3 Expression of the otic anterior marker genes <italic>hmx3a</italic>, <italic>hmx2</italic> and <italic>pax5</italic> after Hh pathway inhibition.

Expression of mRNA for anterior otic markers in embryos treated with 100 μM cyclopamine (cyc) from the 10-somite stage (14 hpf) until 22.5 hpf. Controls in the left-hand panels of each pair of images were treated with vehicle (ethanol) only. (A–F’) At 22.5 hpf (8.5 hours post initiation of treatment, hpt), expression of hmx3a expanded into posterior regions of the otic vesicle (arrowheads); expression of hmx2 showed a modest expansion and there was no change in the otic expression pattern of pax5. Arrowheads in A–D’ indicate posterior extent of otic expression. (G–L’) At 36 hpf (8.5 hpt + 13.5 h wash), expression of both hmx3a and hmx2 extended into posteroventral regions of the otic epithelium. White arrowheads indicate regions that are normally free of expression in controls; black arrowheads mark ectopic expression in cyclopamine-treated embryos. By 36 hpf, expression of pax5 appeared in a new discrete domain in posteromedial otic epithelium after cyclopamine treatment (L, arrowheads); in a lateral view, the epithelium in posterolateral regions was thicker than normal (K’,L’, brackets). A–L are dorsal views showing both otic vesicles, with anterior to the top; A’–L’ are lateral views with anterior to the left. Scale bars, 50 μm (scale bar in A applies to A–F’; in G applies to G–L; in G’ applies to G’–L’).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hmx2 AB control 26+ somites otic vesicle anterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 Not Detected otic vesicle posterior region ISH
AB chemical treatment by environment: Cyclopamine 26+ somites otic vesicle anterior region ISH
26+ somites otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
hmx3a AB control 26+ somites otic vesicle anterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 Not Detected otic vesicle posterior region ISH
AB chemical treatment by environment: Cyclopamine 26+ somites otic vesicle anterior region ISH
26+ somites otic vesicle posterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
pax5 AB control 26+ somites otic vesicle anterior region ISH
Prim-25 otic vesicle anterior region ISH
AB chemical treatment by environment: Cyclopamine 26+ somites otic vesicle anterior region ISH
Prim-25 otic vesicle anterior region ISH
Prim-25 otic vesicle posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: Cyclopamine 26+ somites inner ear morphogenesis decreased process quality, abnormal
26+ somites otic vesicle posterior region hmx2 expression increased amount, abnormal
26+ somites otic vesicle posterior region hmx3a expression increased amount, abnormal
Prim-25 inner ear morphogenesis decreased process quality, abnormal
Prim-25 otic sensory epithelium postero-lateral region increased thickness, abnormal
Prim-25 otic vesicle posterior region hmx2 expression increased amount, abnormal
Prim-25 otic vesicle posterior region pax5 expression increased amount, abnormal
Prim-25 otic vesicle posterior region hmx3a expression increased amount, abnormal
Prim-25 otic vesicle posterior region pax5 expression mislocalised, abnormal
Prim-25 otic vesicle posterior region hmx3a expression mislocalised, abnormal
Prim-25 otic vesicle posterior region hmx2 expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.