ZFIN ID: ZDB-FIG-190723-1035
Gálvez-Santisteban et al., 2019 - Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 7-13

Figure 7

Blood flow regulates myocardial Erbb2 and BMP signaling through endocardial Notch. (A–F) Confocal imaging of BRE:d2GFP; vmhc:mCherry-NTR hearts at 48 hpt (7 dpf) shows that (B) BRE:d2GFP is activated after ventricular ablation (n = 21/21) when compared to (A) uninjured control hearts (n = 0/29); however, (D) blebbistatin (Blebb) (n = 0/11) or (F) DAPT (n = 0/14) treatment inhibits this BRE:d2GFP injury-induced activation. (G–R) Whole-mount in situ hybridizations reveal that bmp10 and nrg1 expression are increased in vmhc:mCherry–NTR (H, N) ventricle-ablated hearts (n = 13/14 bmp10; 7/8 nrg1) at 48 hpt (7 dpf) when compared to (G, M) control uninjured hearts (n = 0/18 bmp10; 0/13 nrg1), while treatment with (J, P) blebbistatin (n = 2/13 bmp10; 0/6 nrg1) or (L, R) DAPT (n = 4/16 bmp10; 0/7 nrg1) inhibits the injury-induced activation of bmp10 and nrg1. All confocal images shown are maximum intensity projections. V, ventricle; A, atrium; dpf, days post fertilization; hpt hours post-MTZ/DMSO treatment. Dashed lines outline the heart. Bars: 50 μm. The following figure supplements are available for Figure 7.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp10 s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole Days 7-13 regenerating tissue ISH
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: blebbistatin Days 7-13 regenerating tissue ISH
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: DAPT Days 7-13 regenerating tissue ISH
d2EGFP mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole Days 7-13 regenerating tissue IFL
mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: blebbistatin Days 7-13 regenerating tissue IFL
mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: DAPT Days 7-13 regenerating tissue IFL
nrg1 s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole Days 7-13 regenerating tissue ISH
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: blebbistatin Days 7-13 regenerating tissue ISH
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: DAPT Days 7-13 regenerating tissue ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole Days 7-13 regenerating tissue d2EGFP expression increased amount, abnormal
mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: blebbistatin Days 7-13 regenerating tissue d2EGFP expression amount, ameliorated
mw30Tg ; s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: DAPT Days 7-13 regenerating tissue d2EGFP expression amount, ameliorated
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole Days 7-13 regenerating tissue nrg1 expression increased amount, abnormal
Days 7-13 regenerating tissue bmp10 expression increased amount, abnormal
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: blebbistatin Days 7-13 regenerating tissue bmp10 expression amount, ameliorated
Days 7-13 regenerating tissue nrg1 expression amount, ameliorated
s957Tg chemical ablation: cardiac ventricle, chemical treatment by environment: metronidazole, chemical treatment by environment: DAPT Days 7-13 regenerating tissue bmp10 expression amount, ameliorated
Days 7-13 regenerating tissue nrg1 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife