Fig. 5

Prostaglandin signaling acts downstream of etv5a during MCC genesis. (A) Schematic of the etv5a locus with locations of the splice MO (blue arrowhead) and the etv5a^sa16031mutation (red arrowhead). The forward arrow in exon 2 depicts the ATG start site. (B) WISH at the 28 ss on representative genotype-confirmed WT embryos, etv5a morphants, and etv5a morphants treated with dmPGE₂. Embryos are imaged in a lateral view, and the Insets correspond to a dorsal view of the pronephros. (Scale bar, 50 μm.) (C) Quantification of odf3b⁺ cells at the 28 ss. Each dot represents the total number of maturing renal MCCs in one embryo. Data are represented as mean ± SEM; significance was determined by ANOVA. (D) Lateral view of the etv5a domain (black line) in the pronephros of representative WT and cox1/2 morphants. (Scale bar, 50 μm.) (E) Quantification of absolute etv5a length in WT versus cox1/2 morphants. Each dot represents the etv5a domain length in one nephron. Data are represented as mean ± SEM; significance was determined by Student’s t test, where *P < 0.0001, **P < 0.006, n.s., not significant.