Normal development of the pronephric tubules and glomerulus in nup133 morphants. (a) Lateral view of pax2a mRNA expression in pronephric tubules at 24 hpf in uninjected (control) and nup133 MOinjected embryos reveal that the developmental expression of pax2a is not altered in nup133 morphants. Twofold magnification of the pronephric tubules is also shown. (b) Pronephros marker cdh17 mRNA expression in nup133 MO is comparable to that of uninjected controls at 24 and 72 hpf (dorsal view). (c) Glomerular development in control and nup133 MO embryos visualized using the podocyte differentiation marker wt1a (dorsal view). At 30 hpf (upper panels), wt1a marks future podocytes with two distinct domains in both control and nup133 MO. At 48 hpf (middle panels), the glomerular primordia merge to the midline to form a single glomerulus. At these stages, mRNA expression does not differ between control and nup133 MO (middle panels). At 58 hpf (lower panels), after the onset of glomerular filtration, the increased area labeled by the wt1a probe (arrow) reflects the glomerular expansion observed in 7 out of 13 nup133 morphants analyzed. Scale bars, 500 μm.
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