Fig. 5

WISH distribution analysis of zic3 transcripts at the shield and 12 hpf stages in embryos incubated with ERK inhibitors. WT (A), vehicle control (B), UO126-treated (C), and SB203580-treated (D) embryos at the shield stage. WT (E), vehicle control (F), UO126-treated (G), and SB203580-treated (H) embryos at 12 hpf. WISH analysis of zic3 transcripts was performed after fixation. Shield-stage embryos are shown from the animal-pole view and 12 hpf embryos are shown from the dorsal view. Red arrowheads show the changes in zic3 expression in embryos treated with ERK inhibitors (SB203580 and UO126) compared with WT embryos. The embryonic shield region is shown in a shield-stage embryo with a red arrowhead. The posterior diencephalon, dorsal spinal cord, and tail bud are shown for the vehicle control in the 12 hpf embryos. (n = 3). Scale bar- 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)