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Pparα deficiency did not affect the formation of neural progenitors. A: GW6471 treatment or injection of pparaa ΔLBD or pparab ΔLBD reduced the expression of acox1, as examined by quantitative real‐time polymerase chain reaction (RT‐qPCR). B: Schematic of pparaa ΔLBD and pparab ΔLBD deletion constructs. DBD, DNA‐binding domain; LBD, ligand‐binding domain. C–J: GW6471 treatment (C,D), injection of pparaa ΔLBD or pparab ΔLBD, and coinjection of pparaa ΔLBD and pparab ΔLBD (E,F) did not alter the expression of neural progenitor marker sox2 and the anterior neural plate marker otx2b, as examined using whole‐mount in situ hybridization (C,E,G,I) and quantified through RT‐qPCR (D,F,H,J). Note that the expression of sox2 at 72 hpf was not affected by Pparα deficiency (G–J). The data represent three independent experiments. Values are expressed as the mean ± standard deviation. **P < 0.01; n.s. not significant. Staging of the embryos is shown in the bottom left corner of each panel in C and E.
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