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ZFIN ID: ZDB-FIG-190129-18
Dingare et al., 2018 - The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish. Development (Cambridge, England)   145(22) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 5

Taz is enriched in the MC precursor before detectable morphological changes. (A-C) Single plane confocal images of cryosectioned ovaries showing E-cadherin (A, n=8) or β-catenin (B,C, n=10) localization in the MC and the neighboring FCs at the contact point (white arrowhead), at the MC membranes (white arrows) and at the interface between the oocyte microvilli and the FCs (yellow arrows). White, cyan and yellow brackets indicate the oocyte cortex, the microvilli/vitelline membrane and the FCL, respectively. Insets (B,C) are higher magnification views of the contact point. Rhodamine Phalloidin (RhPh) and DAPI were used to independently mark membranes and nuclei, respectively. (D-S) Confocal images of wwtr1+/+, wwtr1+/− or wwtr1−/− whole-mount follicles at the indicated stage stained with Taz and/or β-catenin antibodies, ccnb1 mRNA and DAPI as indicated. D,H,L,P are overview images with the follicle size indicated in the lower right corner. White arrowheads in E and Q point to β-catenin enrichment at the contact point; white arrows indicate an MC. E-stage III, early stage III. Scale bars: 10 μm in A-C,E-G,I-K,M-O,Q-S; 50 μm in D,H,L,P.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnb1 WT standard conditions Adult oocyte stage II anterior region ISH
Adult oocyte stage III anterior region ISH
wwtr1fu55/+ standard conditions Adult oocyte stage III anterior region ISH
wwtr1fu55/fu55 standard conditions Adult oocyte stage III anterior region ISH
wwtr1 Ab1-wwtr1 WT standard conditions Adult oocyte stage II micropylar cell IHC
Ab1-wwtr1 Adult oocyte stage III micropylar cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC WT standard conditions Adult micropylar cell apical part of cell
IHC Adult micropylar cell cytoplasm
IHC Adult oocyte stage III micropylar cell
IHC wwtr1fu55/+ standard conditions Adult micropylar cell apical part of cell
IHC Adult ovarian follicle lateral plasma membrane
IHC wwtr1fu55/fu55 standard conditions Adult ovarian follicle lateral plasma membrane
Ab2-cdh1 IHC WT standard conditions Adult micropylar cell apical part of cell
IHC Adult micropylar cell cytoplasm
IHC Adult oocyte stage III micropylar cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development