Eggs from wwtr1?/? females do not form a micropyle. (A-B?) The micropyle is stained by Coomassie Brilliant Blue in activated eggs from wwtr1+/+ females (A,A?, n=10) but not from wwtr1?/? females (B,B?, n=8). Arrows indicate the micropyle. (C-E) Blastula (arrowhead) or partially cleaved (black arrows) eggs from wwtr1+/? (C) or wwtr1?/? (D) females fertilized in vitro after enzymatic removal of the chorion and corresponding quantification (E). (F-I) WISH with the Balbiani body marker dazl (F-I, red arrow) and the animal pole marker ccnb1 (H,I, green arrow) in wwtr1+/? (F,H, n=39) and wwtr1?/? (G, I, n=31) oocytes at the indicated stage of oogenesis. FC nuclei are stained with DAPI. AP, animal pole; VP, vegetal pole. Scale bars: 50??m in F-I; 500 ?m in A-D.
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