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ZFIN ID: ZDB-FIG-190129-17
Dingare et al., 2018 - The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish. Development (Cambridge, England)   145(22) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4

Taz protein is a bona fide marker of the MC. (A-E) Confocal images of stage III WT oocytes stained with anti-Taz (A) and anti-β-catenin (C) antibodies, and DAPI (B, n=4). D shows the three channels merged, and E is an orthogonal view of D. (F-G′) Stage III buc mutant oocytes stained with the anti-Taz antibody and DAPI (n=4). G and G′ are orthogonal views of F. (H-P) Confocal images of wholemount Tg(4xGTIIC:d2egfp)mw50/0 oocytes stained with the anti-Taz antibody (magenta), ccnb1 mRNA (red) and DAPI (blue) at early stage II (H-J), mid-stage II (K-M) and late stage II (N-P) (n=38). Hippo reporter activity is shown in green. Cyan brackets in orthogonal views (M,P) indicate the gap between the oocyte surface and the FC layer (microvilli are not visible); white arrows indicate an MC; dotted arrowheads (I,L) point to non-follicle cells. Insets in L,L′,O,O′ show higher magnification of center of panel. Scale bars: 20 μm in A-D,F,I,J,L,M,O,P; 40 μm in H,K,N.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnb1 mw50Tg standard conditions Adult oocyte stage II anterior region ISH
Adult oocyte stage III anterior region ISH
d2EGFP mw50Tg standard conditions Adult oocyte stage II micropylar cell IHC
Adult oocyte stage III micropylar cell IHC
wwtr1 Ab1-wwtr1 WT standard conditions Adult micropylar cell IHC
Ab1-wwtr1 bucp106re/p106re standard conditions Adult micropylar cell IHC
Ab1-wwtr1 mw50Tg standard conditions Adult oocyte stage II micropylar cell IHC
Ab1-wwtr1 Adult oocyte stage III micropylar cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC WT standard conditions Adult ovarian follicle lateral plasma membrane
IHC bucp106re/p106re standard conditions Adult ovarian follicle lateral plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
bucp106re/p106re standard conditions Adult micropylar cell mislocalised, abnormal
Adult ovarian follicle stage III has extra parts of type micropylar cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development