FIGURE

Fig. 3

ID

ZDB-FIG-181206-28

Publication

Barrodia et al., 2018 - EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

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Fig. 3

Characterizing the phenotype caused by Efhc2 knock-down. a and b WISH for pronephros segment specific genes on 48 hpf morpholino injected embryos. Expression of slc20a1a (PCT), trpm7 (PST), slc12a1 (DE), slc12a3 (DL) and stc1 (CS) in efhc2 mismatch control (efhc2-MM) and splice-blocking morpholino (efhc2-Mo) injected embryos. The phenotype caused by Efhc2 knock-down was rescued by co-injecting efhc2 mRNA along with efhc2 morpholino. c Schematic representation of defects caused by Efhc2 knock-down

Expression Data

Genes:	slc12a1 slc12a3 slc20a1a stc1 trpm7
Fish:	WT WT + MO1-efhc2
Knockdown Reagent:	MO1-efhc2
Anatomical Terms:	corpuscles of Stannius pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-efhc2
Knockdown Reagent:	MO1-efhc2
Observed In:	corpuscles of Stannius pronephric distal early tubule pronephric distal late tubule specification of pronephric distal tubule identity specification of pronephric proximal tubule identity
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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