ZFIN ID: ZDB-FIG-181114-6
Sun et al., 2018 - Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation. Development (Cambridge, England)   145(19) Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 1k-cell to Shield

Fig. 5

Loss of maternal Ybx1 elevates global translation level. (A) Schematic of the fluorescent reporter translation assay. (B,C) Fluorescent images showing GFP reporter levels with rhodamine (Rho) control levels in WT, Mybx1, cMO- and yMO-injected live embryos at indicated stages. Scale bar: 200 μm. (D) Measurement of GFP reporter intensities relative to Rho. (E) Schematic of reporter translation assays using western blotting and luciferase assay. (F) Western blotting analysis of GFP reporter levels at 6 hpf. (G) Measurement of Fluc activities relative to Rluc. (H) SDS-PAGE and silver staining showing global protein levels in embryos. Three regions for measurement are indicated. 3 hpf embryos were lysed for the SDS-PAGE. GFP protein was injected at the one-cell stage as the loading control. (I) Quantification of relative signal intensities of three regions from H. (J) Western blot for the puromycin incorporation assay. GFP protein was injected as the loading control. Gapdh levels are also shown. Un, untreated; Puro, puromycin; CHX, cycloheximide. (K) Puromycin incorporation levels in WT and Mybx1 embryos shown by western blotting. The injected GFP protein served as the loading control. (L) Relative puromycin signal intensities of three regions from western blots in K. *P<0.05, **P<0.01, ***P<0.001; ns, not significant; n=8 embryos for D, n=3 biological replicates for F,G,I,L; Student's t-test.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-ybx1 control 1k-cell translation increased occurrence, abnormal
Shield translation increased occurrence, abnormal
ybx1tsu3d5i/+(TU) control 1k-cell translation increased occurrence, abnormal
Shield translation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development