PUBLICATION

Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation

Authors
Sun, J., Yan, L., Shen, W., Meng, A.
ID
ZDB-PUB-180824-2
Date
2018
Source
Development (Cambridge, England)   145(19): (Journal)
Registered Authors
Meng, Anming
Keywords
Embryogenesis, Maternal-to-zygotic transition, Oocyte maturation, Translational repression, Ybx1, Zebrafish
Datasets
GEO:GSE108924
MeSH Terms
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Models, Biological
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Mutation/genetics
  • Zygote/metabolism*
  • Fertilization
  • Gene Expression Regulation, Developmental
  • Cell Differentiation*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Cell Proliferation
  • Base Sequence
  • Phenotype
  • Animals
  • Female
  • Unfolded Protein Response
  • Oocytes/cytology*
  • Oocytes/metabolism*
  • Y-Box-Binding Protein 1/genetics
  • Y-Box-Binding Protein 1/metabolism*
  • Genetic Pleiotropy
  • Protein Biosynthesis*
(all 26)
PubMed
30135188 Full text @ Development
Abstract
Maternal mRNAs and proteins dictate early embryonic development before zygotic genome activation. In the absence of transcription, elaborate control of maternal mRNA translation is of particular importance for oocyte maturation and early embryogenesis. By analyzing zebrafish ybx1 mutants with a null allele, we demonstrate an essential role of maternal ybx1 in repressing global translation in oocytes and embryos. Loss of maternal Ybx1 leads to impaired oocyte maturation and egg activation. Maternal ybx1 (Mybx1) mutant embryos fail to undergo normal cleavage and the maternal-to-zygotic transition (MZT). Morpholino knockdown of ybx1 also results in MZT loss and epiboly failure, suggesting the postfertilization requirement of Ybx1. In addition, elevated global translation level and the unfolded protein response were found in Ybx1-depleted embryos. Supplementing translational repression by eIF4E inhibition markedly rescues the Mybx1 phenotype. Mechanistically, Ybx1 in embryos may associate with processing body components and repress translation when tethered to target mRNAs. Collectively, our results identify maternal Ybx1 as a global translational repressor required for oocyte maturation and early embryogenesis.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
TU + MO2-ybx1control1k-cell
TU + MO2-ybx1controlShield
TU + MO2-ybx1standard conditions64-cell to Bud
TU + MO2-ybx1standard conditions64-cell to Bud
TU + MO2-ybx1standard conditionsShield
TU + MO2-ybx1standard conditionsShield
TU + MO2-ybx1standard conditionsShield
TU + MO2-ybx1standard conditionsShield
TU + MO2-ybx1standard conditionsShield
TU + MO2-ybx1standard conditionsShield
1 - 10 of 111
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sa42
    		Point Mutation
    tsu3d5i
      		Indel
      tsu29TgTransgenic Insertion
        tsu30TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          ybx1CRISPR3-ybx1CRISPR
          ybx1MO1-ybx1MRPHLNO
          ybx1MO2-ybx1MRPHLNO
          1 - 3 of 3
          Show
          Fish
          Fish
          tsu29Tg + MO2-ybx1 (TU)
          TU
          TU + MO2-ybx1
          ybx1tsu3d5i/+; tsu29Tg (TU)
          ybx1tsu3d5i/+; tsu30Tg (TU)
          ybx1tsu3d5i/+ (TU)
          ybx1tsu3d5i/tsu3d5i (TU)
          1 - 7 of 7
          Show
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          1 - 1 of 1
          Show
          Mapping
          No data available
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          Citation
          Sun, J., Yan, L., Shen, W., Meng, A. (2018) Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation. Development (Cambridge, England). 145(19):.