ZFIN ID: ZDB-FIG-181114-5
Sun et al., 2018 - Maternal Ybx1 safeguards zebrafish oocyte maturation and maternal-to-zygotic transition by repressing global translation. Development (Cambridge, England)   145(19) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Sphere
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 64-cell to Bud

Fig. 3

Postfertilization production of Ybx1 is required for embryonic cell proliferation and epiboly initiation. (A) Bright-field images of embryos injected with cMO or yMO. Some yMO-injected embryos underwent yolk collapse and died, thus reducing the number of embryos with the representative phenotypes from 6 hpf to 10 hpf. (B) yMO-injected WT and Tg(ef1α:GFP-ybx1) embryos. Fluorescent images show the GFP-ybx1 transgene expression driven by zebrafish ef1α promoter. (C) Labelling of pH3-positive cells in MO-injected embryos fixed at 4 hpf, with the quantification displayed by box-and-whisker plots in D. *P<0.05; n=9 regions of interest from three embryos; Student's t-test. (E) Confocal z-projections of DAPI staining. Measurement of the nucleus area is presented as mean±s.d. (n=20 nuclei). (F) Quantification of extracted genomic DNAs from equal numbers of cMO or yMO-injected embryos. Each circle represents the value of a sample containing ten embryos. ns, not significant; *P<0.05, **P<0.01; n=3; Student's t-test. Scale bars: 200 μm in A-C; 10 μm in E.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab18-h3 IHC TU standard conditions Sphere blastomere
IHC ybx1tsu3d5i/+(TU) standard conditions Sphere blastomere
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-ybx1 standard conditions 64-cell - Bud epiboly involved in gastrulation with mouth forming second arrested, abnormal
64-cell - Bud epiboly involved in gastrulation with mouth forming second onset quality, abnormal
tsu29Tg + MO2-ybx1(TU) standard conditions Shield epiboly involved in gastrulation with mouth forming second occurrence, ameliorated
Shield epiboly involved in gastrulation with mouth forming second onset quality, ameliorated
Bud epiboly involved in gastrulation with mouth forming second occurrence, ameliorated
Bud epiboly involved in gastrulation with mouth forming second onset quality, ameliorated
ybx1tsu3d5i/+(TU) standard conditions Sphere blastomere ab18-h3 labeling decreased amount, abnormal
Sphere cell cycle arrested, abnormal
Sphere cell population proliferation process quality, abnormal
30%-epiboly whole organism double-stranded DNA fragment decreased amount, abnormal
Sphere whole organism double-stranded DNA fragment decreased amount, abnormal
Shield whole organism double-stranded DNA fragment decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development