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ZFIN ID: ZDB-FIG-180911-39
Oosterhof et al., 2018 - Colony-Stimulating Factor 1 Receptor (CSF1R) Regulates Microglia Density and Distribution, but Not Microglia Differentiation In Vivo. Cell Reports   24:1203-1217.e6 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 1

Microglia Numbers during Development Are csf1r Dosage-Dependent

(A) Counts per million (CPM) expression values of csf1ra and csf1rb from our previous RNA sequencing study in adult zebrafish microglia (Oosterhof et al., 2017).

(B) Schematic representation of the csf1rb mutation introduced with TALEN-mediated genome editing.

(C and D) 5 days post fertilization (dpf), WT, csf1ra−/−, csf1rb−/−, csf1ra−/−;b+/−, and csf1rDM larvae were treated with neutral red for 2.5 hr. Images were acquired with a stereomicroscope, and microglia numbers were determined by counting the number of neutral red dots. n is at least 15 zebrafish/genotype for (C) and at least 7 for (D).

(E) 4 dpf, WT, csf1ra−/−, csf1ra−/−;b+/− and csf1rDM were labeled with an antibody against L-plastin (Spangenberg et al., 2016), and L-plastin-positive cells were quantified in the optic tecti. n is at least 6 zebrafish/genotype.

LBD, ligand-binding domain; TMD, transmembrane domain; TKD, tyrosine kinase domain. Error bars represent SD. ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA, Bonferroni multiple testing correction). Scale bar, 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra AB control Adult microglial cell RTPCR
csf1rb AB control Adult microglial cell RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-lcp1 IHC AB control Day 4 optic tectum microglial cell
IHC csf1raj4e1/j4e1 control Day 4 optic tectum microglial cell
IHC csf1raj4e1/j4e1 ; csf1rbre01/+ control Day 4 optic tectum microglial cell
IHC csf1rbre01/re01 ; csf1raj4e1/j4e1 control Day 4 optic tectum microglial cell
Phenotype Details
Fish Conditions Stage Phenotype
csf1raj4e1/+ control Day 5 microglial cell decreased amount, abnormal
csf1raj4e1/j4e1 control Day 4 optic tectum microglial cell decreased amount, abnormal
Day 5 microglial cell decreased amount, abnormal
csf1raj4e1/j4e1 ; csf1rbre01/+ control Day 4 optic tectum microglial cell decreased amount, abnormal
Day 5 microglial cell amount, normal
csf1rbre01/re01 control Day 5 microglial cell decreased amount, abnormal
csf1rbre01/re01 ; csf1raj4e1/j4e1 control Day 4 optic tectum microglial cell decreased amount, abnormal
Day 5 microglial cell absent, abnormal
Day 5 microglial cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.