ZFIN Image: Oosterhof et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

Microglia Numbers during Development Are csf1r Dosage-Dependent

(A) Counts per million (CPM) expression values of csf1ra and csf1rb from our previous RNA sequencing study in adult zebrafish microglia (Oosterhof et al., 2017).

(B) Schematic representation of the csf1rb mutation introduced with TALEN-mediated genome editing.

(C and D) 5 days post fertilization (dpf), WT, csf1ra^−/−, csf1rb^−/−, csf1ra^−/−;b^+/−, and csf1r^DM larvae were treated with neutral red for 2.5 hr. Images were acquired with a stereomicroscope, and microglia numbers were determined by counting the number of neutral red dots. n is at least 15 zebrafish/genotype for (C) and at least 7 for (D).

(E) 4 dpf, WT, csf1ra^−/−, csf1ra^−/−;b^+/− and csf1r^DM were labeled with an antibody against L-plastin (Spangenberg et al., 2016), and L-plastin-positive cells were quantified in the optic tecti. n is at least 6 zebrafish/genotype.

LBD, ligand-binding domain; TMD, transmembrane domain; TKD, tyrosine kinase domain. Error bars represent SD. ^∗∗p < 0.01, ^∗∗∗p < 0.001 (one-way ANOVA, Bonferroni multiple testing correction). Scale bar, 40 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.