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ZIRC
ZFIN ID: ZDB-FIG-180907-28
Di et al., 2017 - Zebrafish nephrosin helps host defence against Escherichia coli infection.. Open Biology   7(8) Full text @ Open Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3

npsn deficiency does not affect neutrophil number in zebrafish. (a and a′) WISH analysis of lyz expression and quantification of lyz+ cells in the PBI at 3 dpf (29.5 ± 2.3 versus 26.9 ± 2.7) in WT sibling and npsnsmu5 mutant groups. (Mean ± s.e.m., n = 15 in each group, triplicated). Boxes in the lower right corner outline the magnified PBI regions. (b and b′) WISH analysis of mpx expression and quantification of mpx+ cells in the PBI at 3 dpf (45.9 ± 2.9 versus 38.9 ± 2.9) in WT sibling and npsnsmu5 mutant groups. (Mean ± s.e.m., n = 15 in each group, triplicated). Boxes in the lower right corner outline the magnified PBI regions. (c and c′) Sudan Black staining (SB) and quantification of SB+ cells in the PBI at 3 dpf (57.5 ± 2.7 versus 57.4 ± 5.4) in WT sibling and npsnsmu5 mutant groups). (Mean ± s.e.m., n ≥ 10 in each group, triplicated). Statistical significance was determined by unpaired t-test. n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lyz WT control Protruding-mouth neutrophil ISH
npsnsm15/sm15 control Protruding-mouth neutrophil ISH
mpx WT control Protruding-mouth neutrophil ISH
npsnsm15/sm15 control Protruding-mouth neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npsnsm15/sm15 control Protruding-mouth neutrophil amount, normal
Acknowledgments:
ZFIN wishes to thank the journal Open Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Open Biol.