FIGURE

Fig. 3

ID

ZDB-FIG-180907-28

Publication

Di et al., 2017 - Zebrafish nephrosin helps host defence against Escherichia coli infection.

Other Figures

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Fig. 3

npsn deficiency does not affect neutrophil number in zebrafish. (a and a′) WISH analysis of lyz expression and quantification of lyz⁺ cells in the PBI at 3 dpf (29.5 ± 2.3 versus 26.9 ± 2.7) in WT sibling and npsn^smu5 mutant groups. (Mean ± s.e.m., n = 15 in each group, triplicated). Boxes in the lower right corner outline the magnified PBI regions. (b and b′) WISH analysis of mpx expression and quantification of mpx⁺ cells in the PBI at 3 dpf (45.9 ± 2.9 versus 38.9 ± 2.9) in WT sibling and npsn^smu5 mutant groups. (Mean ± s.e.m., n = 15 in each group, triplicated). Boxes in the lower right corner outline the magnified PBI regions. (c and c′) Sudan Black staining (SB) and quantification of SB⁺ cells in the PBI at 3 dpf (57.5 ± 2.7 versus 57.4 ± 5.4) in WT sibling and npsn^smu5 mutant groups). (Mean ± s.e.m., n ≥ 10 in each group, triplicated). Statistical significance was determined by unpaired t-test. n.s., not significant.

Expression Data

Genes:	lyz mpx
Fish:	WT npsn^sm15/sm15
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	npsn^sm15/sm15
Observed In:	neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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