FIGURE

Fig. 1

ID

ZDB-FIG-180907-26

Publication

Di et al., 2017 - Zebrafish nephrosin helps host defence against Escherichia coli infection.

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Fig. 1

Zebrafish npsn is predominantly expressed in neutrophils. (a) The expression pattern of npsn during zebrafish embryogenesis was initially observed at the rostral blood island at 18 hpf, followed by widespread expression across the site of haematopoiesis in zebrafish. (b) Higher expression of npsn in neutrophils when compared with macrophages. Fluorescent cells were sorted from Tg(mpx:EGFP) and Tg(mpeg1:EGFP), and qRT-PCR was performed to detect the npsn expression, which was highly expressed in neutrophils relative to levels observed in macrophages following adjustment for GFP⁻ cells. Macrophage markers (mfap4 and mpeg1) and neutrophil markers (lyz and mpx) were used as indicators to test the purity of the sorted macrophages and neutrophils; ***p < 0.001. #, undetected. (Mean ± s.e.m., n ≥ 200 per experiment, triplicated). (c) Co-staining for npsn mRNA in mpx⁺ cells at the posterior blood island (PBI) in Tg(mpx:EGFP) embryos. Double staining for anti-GFP and npsn WISH was performed in Tg(mpx:EGFP) cells at 3 dpf. Most mpx⁺ neutrophils also expressed npsn mRNA in the PBI.

Expression Data

Genes:	GFP npsn
Fish:	WT gl22Tg i114Tg
Anatomical Terms:	hematopoietic system macrophage neutrophil rostral blood island
Stage Range:	14-19 somites to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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