Zebrafish nephrosin helps host defence against Escherichia coli infection.
- Di, Q., Lin, Q., Huang, Z., Chi, Y., Chen, X., Zhang, W., Zhang, Y.
- Open Biology 7(8): (Journal)
- Registered Authors
- Chen, Xiaohui, Chi, Yali, Di, Qianqian, Huang, Zhibin, Lin, Qing, Zhang, Wenqing, Zhang, Yiyue
- infection, nephrosin, neutrophil, zebrafish
- MeSH Terms
- CRISPR-Cas Systems
- Disease Models, Animal
- Embryo, Nonmammalian/immunology
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/microbiology
- Escherichia coli Infections/genetics
- Escherichia coli Infections/immunology
- Escherichia coli Infections/prevention & control*
- Gene Knockdown Techniques
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- 28835569 Full text @ Open Biol.
Di, Q., Lin, Q., Huang, Z., Chi, Y., Chen, X., Zhang, W., Zhang, Y. (2017) Zebrafish nephrosin helps host defence against Escherichia coli infection.. Open Biology. 7(8).
Neutrophils play important roles in innate immunity and are mainly dependent on various enzyme-containing granules to kill engulfed microorganisms. Zebrafish nephrosin (npsn) is specifically expressed in neutrophils; however, its function is largely unknown. Here, we generated an npsn mutant (npsnsmu5 ) via CRISPR/Cas9 to investigate the in vivo function of Npsn. The overall development and number of neutrophils remained unchanged in npsn-deficient mutants, whereas neutrophil antibacterial function was defective. Upon infection with Escherichia coli, the npsnsmu5 mutants exhibited a lower survival rate and more severe bacterial burden, as well as augmented inflammatory response to challenge with infection when compared with wild-type embryos, whereas npsn-overexpressing zebrafish exhibited enhanced host defence against E. coli infection. These findings demonstrated that zebrafish Npsn promotes host defence against bacterial infection. Furthermore, our findings suggested that npsn-deficient and -overexpressing zebrafish might serve as effective models of in vivo innate immunity.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes