FIGURE

Fig. 1

ID

ZDB-FIG-180822-17

Publication

Lin et al., 2018 - Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca²⁺ Influx in Hair Cells of Zebrafish Larvae.

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Fig. 1

In situ hybridization and immunocytochemistry of the Ca²⁺-sensing receptor (CaSR) in zebrafish larvae. CaSR mRNA was expressed in neuromasts (arrowheads) and ionocytes (arrows) of 3- day-postfertilization (dpf) larvae (A). The sense probe detected no signal in larval skin (B). Confocal images of CaSR antibody-labeled lateral-line neuromasts of 4-dpf larvae (C, a Z-projection). Confocal z-stacks of the same neuromast revealed that the CaSR was expressed in stereocilia (white arrows in C,D) and basolateral membranes (orange arrows in C,E,F). Double immunocytochemical labeling of the CaSR (H) and actin (I) in 4-dpf larvae; the merged image is shown in (G). White arrows indicate the colocalization of both CaSR and actin signals in stereocilia.

Expression Data

Gene:	casr
Antibody:	Ab1-casr
Fish:	AB
Anatomical Terms:	integument ionocyte posterior lateral line neuromast posterior lateral line neuromast basolateral plasma membrane posterior lateral line neuromast stereocilium
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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