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Fig. 1
In situ hybridization and immunocytochemistry of the Ca2+-sensing receptor (CaSR) in zebrafish larvae. CaSR mRNA was expressed in neuromasts (arrowheads) and ionocytes (arrows) of 3- day-postfertilization (dpf) larvae (A). The sense probe detected no signal in larval skin (B). Confocal images of CaSR antibody-labeled lateral-line neuromasts of 4-dpf larvae (C, a Z-projection). Confocal z-stacks of the same neuromast revealed that the CaSR was expressed in stereocilia (white arrows in C,D) and basolateral membranes (orange arrows in C,E,F). Double immunocytochemical labeling of the CaSR (H) and actin (I) in 4-dpf larvae; the merged image is shown in (G). White arrows indicate the colocalization of both CaSR and actin signals in stereocilia.