FIGURE

Fig. 3

ID

ZDB-FIG-180822-18

Publication

Lin et al., 2018 - Role of Calcium-Sensing Receptor in Mechanotransducer-Channel-Mediated Ca²⁺ Influx in Hair Cells of Zebrafish Larvae.

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Fig. 3

Ca²⁺-sensing receptor (CaSR) protein expression in neuromast hair cell of CaSR morpholino oligonucleotide (MO) knockdown larvae. Immunocytochemical staining with CaSR antibodies was conducted in 4-dpf wild-type larvae (A,B), control MO-injected larvae (control-MO; C,D), and CaSR-MO-injected larvae (CaSR-MO; E–H). CaSR signals (green) were detected in the apical (arrows; A,C) and basolateral (arrows; B,D) membranes of hair cells in wild-type and control-MO larvae. In CaSR-MOs, the CaSR signal was weak in the apical and basolateral membranes (E–H). Scanning electron microscopy images revealed the morphologies of L1 neuromat hair bundles in 4-dpf control-MOs and CaSR-MOs (arrowheads; I,K,M). Immunocytochemical staining with S100 antibodies was conducted in 4-dpf control-MOs and CaSR-MOs (J,L,N). The hair cell numbers of L1 neuromasts in the posterior lateral lines were determined (O). Data are presented as mean ± SE. No significant differences were identified (one-way ANOVA and Tukey’s comparison, p < 0.05). Numbers in parentheses are the numbers of neuromasts. One L1 neuromast per larva was examined.

Expression Data

Antibodies:	Ab1-casr Ab1-s100
Fish:	AB AB + MO2-casr AB + MO3-casr
Knockdown Reagents:	MO2-casr MO3-casr
Anatomical Terms:	posterior lateral line neuromast basolateral plasma membrane posterior lateral line neuromast neuromast hair cell posterior lateral line neuromast stereocilium
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-casr AB + MO3-casr
Knockdown Reagents:	MO2-casr MO3-casr
Observed In:	posterior lateral line neuromast basolateral plasma membrane posterior lateral line neuromast neuromast hair cell posterior lateral line neuromast stereocilium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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