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ZFIN ID: ZDB-FIG-180802-15
Spead et al., 2018 - Characterization of the caspase family in zebrafish. PLoS One   13:e0197966 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4

Fig. 7

Spatial expression of the caspase-8 family members casp8a, casp10 and casp20 at 24, 48, 72 and 96 hpf.

Lateral views of whole embryos stained for casp8a (A-D), casp10 (E-H) and casp20 (I-L) by ISH. Casp8a is expressed in the hindbrain (hb), muscles (ms) and at the floorplate (fp) at 24 hpf (A). Its expression is then strongly detected in the retina (r), diencephalon (di), midbrain (mb), hindbrain and muscles (ms) at 48 hpf (B). Additional expression in the intestinal bulb (ib) is observed at 72 and 96 hpf (C, D). Casp10 expression is detected at the floorplate at 24 and 48 hpf (E, F) and in the pharyngeal arches (pa) at 48 hpf (F). A strong expression is detected in the pharyngeal arches, muscles and intestinal bulb at 72 hpf (G). Casp10 remains highly detected in the pharyngeal arches and intestinal bulb at 96 hpf (H). Casp20 is strongly expressed in the nervous system (ns), vascular system (vs), proctodeum (pr) and throughout the gut (g) at 24 hpf (I). Its expression becomes restricted to the pharyngeal arches and the intestinal bulb at 48, 72 and 96 hpf (J-L). Scale bar: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
casp8 WT standard conditions Prim-5 floor plate ISH
Prim-5 hindbrain ISH
Prim-5 muscle cell ISH
Long-pec diencephalon ISH
Long-pec hindbrain ISH
Long-pec midbrain ISH
Long-pec muscle cell ISH
Long-pec retina ISH
Protruding-mouth diencephalon ISH
Protruding-mouth hindbrain ISH
Protruding-mouth intestinal bulb ISH
Protruding-mouth midbrain ISH
Protruding-mouth muscle cell ISH
Protruding-mouth retina ISH
Day 4 diencephalon ISH
Day 4 hindbrain ISH
Day 4 intestinal bulb ISH
Day 4 midbrain ISH
Day 4 muscle cell ISH
Day 4 retina ISH
casp10 WT standard conditions Prim-5 floor plate ISH
Long-pec floor plate ISH
Long-pec pharyngeal arch ISH
Protruding-mouth intestinal bulb ISH
Protruding-mouth muscle cell ISH
Protruding-mouth pharyngeal arch ISH
Day 4 intestinal bulb ISH
Day 4 pharyngeal arch ISH
casp20 WT standard conditions Prim-5 gut ISH
Prim-5 nervous system ISH
Prim-5 proctodeum ISH
Prim-5 vasculature ISH
Long-pec intestinal bulb ISH
Long-pec pharyngeal arch ISH
Protruding-mouth intestinal bulb ISH
Protruding-mouth pharyngeal arch ISH
Day 4 intestinal bulb ISH
Day 4 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One