FIGURE

Fig. 2

ID
ZDB-FIG-180723-19
Publication
Fassier et al., 2018 - Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics
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Fig. 2

Fignl1 associates with MTs in spinal motor axons. (A) Immunodetection of Fignl1 (red), acetylated α-tubulin (blue), and GFP (green) in 26-hpf Tg(Hb9:GFP) transgenic embryos. Lateral views of the trunk. (B) GC from cultured Tg(Hb9:GFP) SMN labeled with phalloidin (green), tyrosinated tubulin (blue), and Fignl1 (red) antibodies. Arrows indicate actin protrusions. (C) Detergent extraction of soluble proteins from WT or Tg(Hb9:GFP) spinal neurons immunostained by tyrosinated tubulin (red) and Fignl1 or GFP (green). Bars: (A and B) 10 µm; (C) 20 µm. (D) MT copelleting assay from COS-7 cells transfected with pCS2+-HA or pCS2+-HA-Fignl1 (HA-Fignl1). Supernatants (S) were incubated with taxol and GTP to induce MT polymerization and centrifuged on a sucrose cushion to pellet MTs and their associated proteins (P2). Soluble tubulins and proteins that did not bind MTs remained in the S2 supernatant. WBs assessed Fignl1 and α-tubulin levels in different fractions. L, total lysate.

Expression Data
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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