FIGURE

Fig. S3

ID

ZDB-FIG-180723-28

Publication

Fassier et al., 2018 - Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Fignl1 depletion does not perturb the specification, localization, or survival of SMNs. (A) Immunolabeling of SMNs (MNs), RB sensory neurons, and GABAergic interneurons in 26-hpf MO^Ctl and MO^Fignl1 embryos with Islet1/2 (green) and GABA (red) antibodies. Lateral views of the trunk; anterior is to the left. Bar, 50 μm. (B and C) Mean number (Nb.) of MNs, RB neurons, and GABAergic interneurons (GABA). Quantifications were performed on 26-hpf control (n = 22) and morphant (n = 21) embryos pooled from three independent experiments. 12 hemisegments were analyzed in the anterior (B) and posterior regions (C) of each embryonic trunk. ***, P ≤ 0.001; unpaired two-tailed t test. Error bars are SEM. (D) Immunolabeling of activated caspase 3 (red) and the neuronal postmitotic marker HuC/D (green; left) or caspase 3 alone (red; right) in 26- (left) or 56-hpf (right) MO^Ctl and MO^Fignl1 embryos and larvae. Insets represent higher magnifications of the indicated boxed regions. Fignl1 knockdown does not induce apoptotic cell death of spinal neurons. Bars, 30 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.