FIGURE

Fig. 2

ID

ZDB-FIG-180712-78

Publication

Esancy et al., 2018 - A zebrafish and mouse model for selective pruritus via direct activation of TRPA1

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Fig. 2

Trpa1b, but not Tlr7, is required for both neuronal and behavioral responses to IMQ.

(A, B) In situ hybridization in 3dpf WT larvae probing for tlr7 and trpa1b mRNA respectively. (C, D) Larval locomotor assay of 5dpf tlr7^+/+ and tlr7^-/- (C) or trpa1b^+/+and trpa1b^-/- (D) larvae. (E, F) Adult lip-rubbing behavioral assay of tlr7^+/+/tlr7^-/- (E) and trpa1b^+/+/trpa1b^-/-(F) fish. (C), (D), (E), (F), 100 μM IMQ used. ***p<0.001, **p<0.01, Student’s t-test. Bars represent mean ± s.e.m. (G, H) Representative calcium imaging traces of 3dpf trpa1b^+/+ (G) and trpa1b^-/- (H) larvae in a transgenic elavl3HuC:GCaMP5 background exposed to 100 μM IMQ, 50 μM AITC, and 1 mM 2-APB. B = blood, TG = trigeminal ganglion.

Expression Data

Genes:	tlr7 trpa1b
Fish:	WT
Anatomical Terms:	blood trigeminal ganglion
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT tlr7^{zf2185/zf2185} trpa1b^{zf2184/zf2184}
Condition:	chemical treatment: imiquimod
Observed In:	chemosensory behavior sensory perception of chemical stimulus swimming
Stage:	Day 5

Phenotype Detail

Acknowledgments

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