ZFIN Image: Esancy et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

Trpa1b, but not Tlr7, is required for both neuronal and behavioral responses to IMQ.

(A, B) In situ hybridization in 3dpf WT larvae probing for tlr7 and trpa1b mRNA respectively. (C, D) Larval locomotor assay of 5dpf tlr7^+/+ and tlr7^-/- (C) or trpa1b^+/+and trpa1b^-/- (D) larvae. (E, F) Adult lip-rubbing behavioral assay of tlr7^+/+/tlr7^-/- (E) and trpa1b^+/+/trpa1b^-/-(F) fish. (C), (D), (E), (F), 100 ?M IMQ used. ***p<0.001, **p<0.01, Student?s t-test. Bars represent mean � s.e.m. (G, H) Representative calcium imaging traces of 3dpf trpa1b^+/+ (G) and trpa1b^-/- (H) larvae in a transgenic elavl3HuC:GCaMP5 background exposed to 100 ?M IMQ, 50 ?M AITC, and 1 mM 2-APB. B = blood, TG = trigeminal ganglion.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife