FIGURE

Fig. S3

ID

ZDB-FIG-180711-69

Publication

Kontarakis et al., 2018 - Mir-126 is a conserved modulator of lymphatic development

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Fig. S3

mir-126 mutants and morphants develop lymphatic hypoplasia. mir-126 mutant larvae display severe edema around the eyes and in the trunk starting at 5 dpf. (A) The lymphatic defects precede the onset of edema. mutant animals exhibit reduced parachordal lymphangioblast (PL) number at 60 hpf and thoracic duct (TD) hypoplasia at 120 hpf. Angiogenic secondary sprouting (vISV number) is not significantly affected. All images are maximal projections of 20–40 confocal sections, lateral views, anterior to the left. Arrows and asterisks indicate presence or absence of the trait of interest, respectively. (B) Quantification of segments with PL at 60 hpf, TD at 120 hpf and vISVs at 120 hpf. Minimum 5 animals per genotype were scored in each experiment per cross; data from> 2 independent crosses. Error bars represent SEM. Unpaired Student’s

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mir126a^bns2/bns2; mir126b^bns3/bns3; ci1Tg
Observed In:	intersegmental vessel sprouting angiogenesis thoracic duct vascular lymphangioblast
Stage Range:	Pec-fin to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.