FIGURE

Fig. 3

ID

ZDB-FIG-180711-67

Publication

Kontarakis et al., 2018 - Mir-126 is a conserved modulator of lymphatic development

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Fig. 3

flt4^+/- larvae are sensitive to mild miR-126 knockdown. (A) Design of the experiment: embryos from a flt4^+/- outcross were injected with a low dose of mir-126 MO, scored for lymphatic defects at 5 dpf, and then genotyped. (B, C) Facial lymphatic analysis of flt4^+/- and wild-type siblings injected with 1 ng of mir-126 MO. Uninjected larvae of both genotypes develop a functional facial lymphatic network. However, unlike their wild-type siblings, flt4^+/- larvae display facial lymphatic defects (asterisk) when injected with 1 ng of mir-126 MO. Graph shows data from 3 independent experiments. Total number of larvae analysed: 73 (35 flt4^+/- and 38 flt4^+/+). (D,E) Thoracic duct (TD) analysis. Representative side views for normal, partial, and no TD development; asterisks indicate lack of TD formation; two suboptimal doses of mir-126 MO (0,5 ng and 1 ng) were used. Wild-type siblings display a largely complete TD; flt4^+/- injected with mir-126 MO resemble mir-126 mutants. Results from 4 independent experiments; 16 larvae analysed per experiment. Unpaired Student's t-test was performed to test for statistical significance.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	flt4^hu4602/+; nz101Tg + MO1-mir126 flt4^hu4602/+; y1Tg + MO1-mir126
Knockdown Reagent:	MO1-mir126
Observed In:	lateral facial lymph vessel lymph vessel development medial facial lymph vessel thoracic duct
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.