ZFIN Image: Kontarakis et al., 2018, Fig. S3

Image

Figure Caption

Fig. S3

mir-126 mutants and morphants develop lymphatic hypoplasia. mir-126 mutant larvae display severe edema around the eyes and in the trunk starting at 5 dpf. (A) The lymphatic defects precede the onset of edema. mutant animals exhibit reduced parachordal lymphangioblast (PL) number at 60 hpf and thoracic duct (TD) hypoplasia at 120 hpf. Angiogenic secondary sprouting (vISV number) is not significantly affected. All images are maximal projections of 20–40 confocal sections, lateral views, anterior to the left. Arrows and asterisks indicate presence or absence of the trait of interest, respectively. (B) Quantification of segments with PL at 60 hpf, TD at 120 hpf and vISVs at 120 hpf. Minimum 5 animals per genotype were scored in each experiment per cross; data from> 2 independent crosses. Error bars represent SEM. Unpaired Student’s

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 437(2), Kontarakis, Z., Rossi, A., Ramas, S., Dellinger, M.T., Stainier, D.Y.R., Mir-126 is a conserved modulator of lymphatic development, 120-130, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.