ZFIN ID: ZDB-FIG-180711-1
Marsden et al., 2018 - A Cyfip2-Dependent Excitatory Interneuron Pathway Establishes the Innate Startle Threshold. Cell Reports   23:878-887 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. S3

Cyfip2 is expressed broadly in neuropil; Tg(hsp70:cyfip2-GFP) is detectable in CNS for approximately 24 hours after heat shock, Related to Figure 2 (A) Cyfip2 Ab stain in cyfip2+/+ and cyfip2p400/p400 larvae at 72 hpf. OB: olfactory bulb, T: tectum, AC: anterior commissure, OC: optic chiasm, IPL: inner plexiform layer, OPL: outer plexiform layer (scale bar 30 μm). (B) Cyfip2 Ab stain (magenta) at 72 hpf showing labeling near the lateral dendrite of the Mauthner cell (M, green), labeled by Tg(GFFDMC130A);Tg(UAS:gap43-citrine) (scale bar: B’-B’’’: 30 μm; B’’’’: 15 μm). (C) Expression of Tg(hsp70:cyfip2-GFP) after a single 40 min heat shock at 37°C in larvae with (bottom) and without (top) the transgene. Scale bars 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cyfip2 Ab1-cyfip2 WT standard conditions Protruding-mouth anterior commissure IHC
Ab1-cyfip2 Protruding-mouth hindbrain IHC
Ab1-cyfip2 Protruding-mouth olfactory bulb IHC
Ab1-cyfip2 Protruding-mouth Not Detected optic chiasm IHC
Ab1-cyfip2 Protruding-mouth optic tectum IHC
Ab1-cyfip2 Protruding-mouth retinal inner plexiform layer IHC
Ab1-cyfip2 Protruding-mouth retinal outer plexiform layer IHC
Ab1-cyfip2 cyfip2p400/p400 standard conditions Protruding-mouth Not Detected hindbrain IHC
Ab1-cyfip2 Protruding-mouth Not Detected optic chiasm IHC
Ab1-cyfip2 Protruding-mouth Not Detected optic tectum IHC
Ab1-cyfip2 Protruding-mouth Not Detected retinal inner plexiform layer IHC
Ab1-cyfip2 Protruding-mouth Not Detected retinal outer plexiform layer IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cyfip2p400/p400 standard conditions Protruding-mouth hindbrain cyfip2 expression absent, abnormal
Protruding-mouth optic tectum cyfip2 expression absent, abnormal
Protruding-mouth retinal inner plexiform layer cyfip2 expression absent, abnormal
Protruding-mouth retinal outer plexiform layer cyfip2 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.