ZFIN ID: ZDB-FIG-180710-57
Marsden et al., 2018 - A Cyfip2-Dependent Excitatory Interneuron Pathway Establishes the Innate Startle Threshold. Cell Reports   23:878-887 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 5

SF Neurons Are Hyperexcited by Weak Acoustic Stimuli in cyfip2 Mutants and Reversal of the Hypersensitivity Phenotype

(A) Representative SF neuron Ca2+ responses in cyfip2 siblings and mutants 1 s before (F0) and 150 ms after (peak) medium-intensity (−12 dB) acoustic stimulation. Dashed circles indicate SF cell bodies, arrowheads mark cells that fired according to our criteria (see text). Scale bar, 10 μm.

(B) Distribution of SF neuron firing probability (n = 48 cells from 8 siblings, 36 cells from 6 mutants; ∗∗∗∗p < 0.0001, Mann-Whitney test).

(C) Startle sensitivity index of cyfip2p400 siblings and mutants expressing Tg(hsp70:cyfip2-GFP) 4 dpf before 8 heat shock cycles at 37°C separated by 120 min (d4 pre; p = 0.025, unpaired t test). The same larvae were tested for startle sensitivity after heat shock at 6 dpf (d6 post; ∗∗p = 0.0018, paired t test). cyfip2p400 mutants without the hsp70:cyfip2-GFP transgene remained hypersensitive (p = 0.46, paired t test).

(D) Model of Cyfip2’s role in the startle circuit. In cyfip2 mutants, activity is enhanced in the VIII-SF-M-cell pathway, either through a direct VIII-SF connection or through an indirect connection via an unknown cell population (question mark), leading to enhanced M-cell firing and startle behavior. In wild-type fish, Cyfip2 potentially acts at pre- and/or postsynaptic sites, indicated by asterisks, to dampen neural activity.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GCaMP a151Tg ; a150Tg standard conditions Day 5 spiral neuron cell body IFL
a151Tg ; a150Tg acoustic radiation Day 5 spiral neuron cell body IFL
a151Tg ; cyfip2p400/p400 ; a150Tg standard conditions Day 5 spiral neuron cell body IFL
a151Tg ; cyfip2p400/p400 ; a150Tg acoustic radiation Day 5 spiral neuron cell body IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
a151Tg ; cyfip2p400/p400 ; a150Tg acoustic radiation Day 5 spiral neuron calcium-mediated signaling increased occurrence, abnormal
Day 5 spiral neuron cell body GCaMP expression increased amount, abnormal
Day 5 spiral neuron membrane depolarization during action potential increased occurrence, abnormal
Day 5 spiral neuron membrane depolarization increased occurrence, abnormal
Day 5 spiral neuron neuronal action potential increased occurrence, abnormal
cyfip2p400/p400 standard conditions Day 4 startle response increased occurrence, abnormal
Day 4 startle response increased sensitivity of a process sensory perception of sound, abnormal
cyfip2p400/p400 heat shock Day 6 startle response increased occurrence, abnormal
Day 6 startle response increased sensitivity of a process sensory perception of sound, abnormal
p407Tg standard conditions Day 4 startle response occurrence, normal
Day 4 startle response sensitivity of a process sensory perception of sound, normal
p407Tg heat shock Day 6 startle response occurrence, normal
Day 6 startle response sensitivity of a process sensory perception of sound, normal
p407Tg ; cyfip2p400/+ standard conditions Day 4 startle response occurrence, normal
Day 4 startle response sensitivity of a process sensory perception of sound, normal
p407Tg ; cyfip2p400/+ heat shock Day 6 startle response occurrence, normal
Day 6 startle response sensitivity of a process sensory perception of sound, normal
p407Tg ; cyfip2p400/p400 standard conditions Day 4 startle response increased occurrence, abnormal
Day 4 startle response increased sensitivity of a process sensory perception of sound, abnormal
p407Tg ; cyfip2p400/p400 heat shock Day 6 startle response occurrence, ameliorated
Day 6 startle response sensitivity of a process sensory perception of sound, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.