ZFIN ID: ZDB-FIG-180710-54
Marsden et al., 2018 - A Cyfip2-Dependent Excitatory Interneuron Pathway Establishes the Innate Startle Threshold. Cell Reports   23:878-887 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 4

SF Axon Terminal Activity Is Increased in cyfip2 Mutants

(A) Maximum intensity projection of Tg(−6.7FRhcrtR:gal4VP16); Tg(UAS:GCaMP5), showing labeled SF neurons (green). M-cells (M) and other reticulospinal neurons were labeled with rhodamine dextran (magenta). Arrowheads indicate SF cell bodies, and asterisks mark SF axon terminals in the M-cell axon cap. Scale bar, 10 μm.

(B) Representative pseudocolored images of baseline (F0) and peak fluorescence in SF axon terminals following a strong acoustic stimulus (13 dB; the color scale denotes fluorescence intensity; black, lowest; white, highest). Scale bar, 10 μm.

(C) Averaged traces of SF terminal Ca2+ responses following low (−14 dB), medium (−12 dB), and strong (13 dB) acoustic stimuli (n = 42 responses from 10 siblings, blue line; n = 36 responses from 9 mutants, red line; mean ± SEM; p < 0.05, Mann-Whitney test).

(D) Scatterplot of the area under the curve for individual SF axon terminal Ca2+ responses (mean ± SD; ∗∗p = 0.0049, ∗∗∗p = 0.0003, Mann-Whitney test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GCaMP a151Tg ; a150Tg acoustic radiation Day 5 spiral neuron axon terminus IFL
a151Tg ; cyfip2p400/p400 ; a150Tg acoustic radiation Day 5 spiral neuron axon terminus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
a151Tg ; cyfip2p400/p400 ; a150Tg acoustic radiation Day 5 spiral neuron axon terminus GCaMP expression increased amount, abnormal
Day 5 spiral neuron calcium-mediated signaling increased occurrence, abnormal
Day 5 spiral neuron membrane depolarization increased occurrence, abnormal
Day 5 startle response increased sensitivity of a process sensory perception of sound, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.