ZFIN ID: ZDB-FIG-180709-44
Andrzejczuk et al., 2018 - Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons. Frontiers in neuroscience   12:170 Full text @ Front. Neurosci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 9

Expression of Tg(-8.1gata1:gata1-EGFP) in single and double mutants. Lateral views of 30 h Tg(-8.1gata1:gata1-EGFP) WT sibling (A) and single and double mutants as indicated (B–F). Dorsal, top; anterior, left. Dashed rectangle regions in main panels (A–F) are shown in magnified view in adjacent (A–F) panels. Dashed lines indicate ventral limit of spinal cord. White arrows indicate KA″ axons, magenta arrows indicate KA′ axons and white arrowheads mark V2b axons. KA″ and KA′ axons are ipsilateral and ascending whereas V2b axons are ipsilateral descending. KA″ neurons appear to form normally and extend WT-like axonal projections in tal1;gata3 double mutants (E,E). In contrast, even though GFP-expressing V2b cells are present in both gata3 (C,C) and gata2a (D,D) single mutants there are no GFP-expressing V2b cells in gata2a;gata3 double mutants (F,F). Scale bar = 50 microns.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata3sa0234/sa0234 ; zf100Tg standard conditions Prim-5 Kolmer-Agduhr neuron ventral-most region morphology, normal
Prim-5 spinal cord has normal numbers of parts of type Kolmer-Agduhr neuron ventral-most region, normal
Prim-5 spinal cord has normal numbers of parts of type VeLD, normal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron medial region, abnormal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron ventro-medial region, abnormal
Prim-5 VeLD morphology, normal
tal1t21384/t21384 ; gata3sa0234/sa0234 ; zf100Tg standard conditions Prim-5 Kolmer-Agduhr neuron ventral-most region morphology, normal
Prim-5 spinal cord has normal numbers of parts of type Kolmer-Agduhr neuron ventral-most region, normal
zf100Tg standard conditions Prim-5 spinal cord has normal numbers of parts of type interneuron, normal
zf100Tg ; gata2aum27/um27 standard conditions Prim-5 Kolmer-Agduhr neuron medial region morphology, normal
Prim-5 Kolmer-Agduhr neuron ventral-most region morphology, normal
Prim-5 Kolmer-Agduhr neuron ventro-medial region morphology, normal
Prim-5 spinal cord has fewer parts of type Kolmer-Agduhr neuron ventral-most region, abnormal
Prim-5 spinal cord has normal numbers of parts of type Kolmer-Agduhr neuron ventral-most region, normal
Prim-5 spinal cord has normal numbers of parts of type VeLD, normal
zf100Tg ; gata3sa0234/sa0234 ; gata2aum27/um27 standard conditions Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron medial region, abnormal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron ventral-most region, abnormal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron ventro-medial region, abnormal
Prim-5 spinal cord lacks all parts of type VeLD, abnormal
zf100Tg ; tal1t21384/t21384 standard conditions Prim-5 Kolmer-Agduhr neuron ventral-most region morphology, normal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron medial region, abnormal
Prim-5 spinal cord lacks all parts of type Kolmer-Agduhr neuron ventro-medial region, abnormal
Prim-5 VeLD absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neurosci.