ZFIN ID: ZDB-FIG-180709-47
Andrzejczuk et al., 2018 - Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons. Frontiers in neuroscience   12:170 Full text @ Front. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 12

Phospho-histone H3 (pH3) immunohistochemistry in tal1, gata3, and gata2a mutants. Lateral views of pH3 (red) and GFP (green) immunohistochemistry in 24 h Tg(-8.1gata1:gata1-EGFP) WT sibling and mutant embryos as indicated (A,B,D,E,G,H). Top panel (1) in each case shows just the red channel and bottom panel (2) shows the merged image of both green and red channels. Tg(-8.1gata1:gata1-EGFP) embryos were used to help orientate to particular rows/cell type regions in the ventral spinal cord. Dorsal, top; anterior, left. Scale bar = 50 microns. Dashed lines indicate ventral limit of spinal cord. Mean number of pH3-positive cells in each D/V row of spinal cord region adjacent to somites 6–10 in WT and mutant embryos (C,F,I). All counts are an average of at least 3 embryos. Cell rows were assigned based on average cell diameters. Error bars indicate SEM. Statistically significant comparisons are indicated with brackets and stars. *P < 0.05, **P < 0.01. For P-values see Supplementary Table 6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gata3sa0234/sa0234 ; zf100Tg standard conditions Prim-5 Kolmer-Agduhr neuron IFL
zf100Tg standard conditions Prim-5 Kolmer-Agduhr neuron IFL
zf100Tg ; gata2aum27/um27 standard conditions Prim-5 Kolmer-Agduhr neuron IFL
Prim-5 primary motor neuron IFL
zf100Tg ; tal1t21384/t21384 standard conditions Prim-5 Kolmer-Agduhr neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata3sa0234/sa0234 ; zf100Tg standard conditions Prim-5 spinal cord interneuron cell division increased occurrence, abnormal
zf100Tg standard conditions Prim-5 spinal cord interneuron cell division occurrence, normal
zf100Tg ; gata2aum27/um27 standard conditions Prim-5 spinal cord interneuron cell division occurrence, normal
zf100Tg ; tal1t21384/t21384 standard conditions Prim-5 spinal cord interneuron cell division increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neurosci.