FIGURE

Fig. 12

ID

ZDB-FIG-180709-47

Publication

Andrzejczuk et al., 2018 - Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid-Contacting KA Spinal Neurons

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Fig. 12

Phospho-histone H3 (pH3) immunohistochemistry in tal1, gata3, and gata2a mutants. Lateral views of pH3 (red) and GFP (green) immunohistochemistry in 24 h Tg(-8.1gata1:gata1-EGFP) WT sibling and mutant embryos as indicated (A,B,D,E,G,H). Top panel (1) in each case shows just the red channel and bottom panel (2) shows the merged image of both green and red channels. Tg(-8.1gata1:gata1-EGFP) embryos were used to help orientate to particular rows/cell type regions in the ventral spinal cord. Dorsal, top; anterior, left. Scale bar = 50 microns. Dashed lines indicate ventral limit of spinal cord. Mean number of pH3-positive cells in each D/V row of spinal cord region adjacent to somites 6–10 in WT and mutant embryos (C,F,I). All counts are an average of at least 3 embryos. Cell rows were assigned based on average cell diameters. Error bars indicate SEM. Statistically significant comparisons are indicated with brackets and stars. *P < 0.05, **P < 0.01. For P-values see Supplementary Table 6.

Expression Data

Gene:	EGFP
Fish:	zf100Tg gata2a^um27/um27; zf100Tg gata3^{sa0234/sa0234}; zf100Tg tal1^{t21384/t21384}; zf100Tg
Anatomical Terms:	Kolmer-Agduhr neuron primary motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf100Tg gata2a^um27/um27; zf100Tg gata3^{sa0234/sa0234}; zf100Tg tal1^{t21384/t21384}; zf100Tg
Observed In:	spinal cord interneuron cell division
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.