FIGURE

Fig. 2

ID

ZDB-FIG-180705-55

Publication

Williams et al., 2018 - Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes

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Fig. 2

MZudu mutant gastrulae exhibit irregular notochord boundaries. a–b Still images from live Nomarski time-lapse series of the dorsal mesoderm in WT (a) and MZudu−/− embryos (b) at the time points indicated. Images are representative of over 40 MZudu−/− gastrulae. Arrowheads indicate notochord boundaries. c–d Live confocal microscope images of representative WT (c, N = 23) and MZudu−/− (d, N = 43) embryos expressing membrane Cherry. Yellow lines mark notochord boundaries. e Quantification of notochord boundary straightness in live WT and MZudu−/− gastrulae throughout gastrulation. Symbols are means with SEM (two-way ANOVA, ****p < 0.0001). f–g Confocal microscope images of immunofluorescent staining for pan-Laminin in WT (f) and MZudu−/− (g) embryos at two-somite stage. N indicates the number of embryos analyzed. Scale bars are 50 μm. Anterior is up in all images

Expression Data

Antibody:	Ab1-lama1
Fish:	AB/TU gon4lb^vu66/vu66
Anatomical Term:	notochord lateral margin
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gon4lb^vu66/vu66
Observed In:	notochord lateral margin notochord morphogenesis
Stage Range:	75%-epiboly to 1-4 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.