PUBLICATION

Gon4l regulates notochord boundary formation and cell polarity underlying axis extension by repressing adhesion genes

Authors
Williams, M.L.K., Sawada, A., Budine, T., Yin, C., Gontarz, P., Solnica-Krezel, L.
ID
ZDB-PUB-180405-9
Date
2018
Source
Nature communications   9: 1319 (Journal)
Registered Authors
Budine, Terin, Sawada, Atsushi, Solnica-Krezel, Lilianna, Williams, Margot, Yin, Chunyue
Keywords
none
Datasets
GEO:GSE96576, GEO:GSE96575
MeSH Terms
  • Animals
  • Body Patterning
  • Cell Adhesion
  • Cell Communication
  • Chromatin/chemistry
  • Crosses, Genetic
  • Erythroid-Specific DNA-Binding Factors/genetics*
  • Erythroid-Specific DNA-Binding Factors/physiology*
  • Gene Expression Regulation, Developmental*
  • Membrane Glycoproteins/physiology
  • Mutation
  • Notochord/physiology
  • Sequence Analysis, RNA
  • Signal Transduction
  • Xenopus
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
PubMed
29615614 Full text @ Nat. Commun.
Abstract
Anteroposterior (AP) axis extension during gastrulation requires embryonic patterning and morphogenesis to be spatiotemporally coordinated, but the underlying genetic mechanisms remain poorly understood. Here we define a role for the conserved chromatin factor Gon4l, encoded by ugly duckling (udu), in coordinating tissue patterning and axis extension during zebrafish gastrulation through direct positive and negative regulation of gene expression. Although identified as a recessive enhancer of impaired axis extension in planar cell polarity (PCP) mutants, udu functions in a genetically independent, partially overlapping fashion with PCP signaling to regulate mediolateral cell polarity underlying axis extension in part by promoting notochord boundary formation. Gon4l limits expression of the cell-cell and cell-matrix adhesion molecules EpCAM and Integrin╬▒3b, excesses of which perturb the notochord boundary via tension-dependent and -independent mechanisms, respectively. By promoting formation of this AP-aligned boundary and associated cell polarity, Gon4l cooperates with PCP signaling to coordinate morphogenesis along the AP embryonic axis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes