ZFIN ID: ZDB-FIG-180613-21
Kasheta et al., 2017 - Identification and characterization of T reg-like cells in zebrafish. The Journal of experimental medicine   214(12):3519-3530 Full text @ J. Exp. Med.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Lymphocyte expansion and lymphocyte-dependent inflammation in foxp3a mutants. (A) Flow cytometry analyses of WKM from representative foxp3a mutant animals. (B) Differential counts of nonerythroid cells from WKM. Counts were obtained from at least 200 cells per WKM cytospin with May-Grünwald Giemsa staining. Two-tailed Student’s t test (foxp3a mutant vs. AB), *, P < 0.05; **, P < 0.001. (C) Representative images of hematoxylin and eosin–stained sagittal sections of spleens from wild-type AB and foxp3a(um252) mutants. Images showing largest extent of splenic size are shown. Spleens are shown bordered by dashed lines. Bars, 250 µm. (D) Representative images of dissected spleens from Tg(lck:EGFP) and Tg(lck:EGFP); foxp3a(um252) animals. Spleens are shown bordered by dotted lines. Bars, 250 µm. (E) Flow cytometry analysis of spleens from AB (n = 5), Tg(lck:EGFP) (n = 5) and Tg(lck:EGFP); foxp3a(um252) (n = 5) animals. Percentages of EGFP-positive cells in the lymphocyte gate are shown. Two-tailed Student’s t test, *, P < 0.05. (F) Representative images of hematoxylin and eosin–stained sagittal sections of urogenital pores from wild-type AB, foxp3a(um252), and foxp3a(um252); rag1(lf) animals. In total, 0/4 wild-type AB, 6/6 foxp3a(um252), and 0/3 foxp3a(um252); rag1(lf) animals displayed connective tissue inflammation. Insets, increased cellularity characterized by marked influx of lymphocyte-predominant inflammatory cells (arrowheads) in foxp3a(um252) mutants compared with the wild-type AB and foxp3a(um252); rag1(lf) double mutants. Bars: 100 µm; (inset) 10 µm. (G) Representative images of hematoxylin and eosin–stained sagittal sections of skin from wild-type AB, foxp3a(um252), and foxp3a(um252); rag1(lf) animals. In total, 0/4 wild-type AB, 5/6 foxp3a(um252), and 0/3 foxp3a(um252); rag1(lf) animals displayed skin inflammation. Bar, 25 µm. (H) qRT-PCR analysis of inflammation marker genes ifng1-2 and il1b. ΔCt values were calculated relative to a β-actin control. Two-tailed Student’s t test, *, P < 0.05; ns, not significant. Error bar indicates SEM; n = 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ifng1 foxp3aum252/um252(AB) standard conditions Adult integument RTPCR
Adult posterior intestine RTPCR
il1b foxp3aum252/um252(AB) standard conditions Adult posterior intestine RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cz2Tg ; foxp3aum252/um252 standard conditions Adult spleen lymphocyte increased amount, abnormal
foxp3aum252/um252 ; rag1hu1999/hu1999 standard conditions Adult cloacal chamber inflammatory response occurrence, ameliorated
foxp3aum252/um252(AB) standard conditions Adult cloacal chamber inflammatory response increased occurrence, abnormal
Adult head kidney lymphocyte increased amount, abnormal
Adult head kidney myeloid cell decreased amount, abnormal
Adult integument ifng1 expression decreased amount, abnormal
Adult posterior intestine ifng1 expression decreased amount, abnormal
Adult posterior intestine il1b expression decreased amount, abnormal
Adult spleen increased size, abnormal
foxp3aum253/+ ; foxp3aum252/+(AB) standard conditions Adult head kidney lymphocyte increased amount, abnormal
Adult head kidney myeloid cell decreased amount, abnormal
foxp3aum253/um253(AB) standard conditions Adult head kidney lymphocyte increased amount, abnormal
Adult head kidney myeloid cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The Journal of experimental medicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Exp. Med.