PUBLICATION
Identification and characterization of T reg-like cells in zebrafish
- Authors
- Kasheta, M., Painter, C.A., Moore, F.E., Lobbardi, R., Bryll, A., Freiman, E., Stachura, D., Rogers, A.B., Houvras, Y., Langenau, D.M., Ceol, C.J.
- ID
- ZDB-PUB-171028-22
- Date
- 2017
- Source
- The Journal of experimental medicine 214(12): 3519-3530 (Journal)
- Registered Authors
- Ceol, Craig, Houvras, Yariv, Langenau, David
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Chronic Disease
- Gene Expression Regulation
- Genes, Reporter
- Green Fluorescent Proteins/metabolism
- Hematopoiesis
- Inflammation/pathology
- Lymphocytes/metabolism
- Mutation/genetics
- Phylogeny
- Splenomegaly/pathology
- Survival Analysis
- T-Lymphocytes, Regulatory/immunology*
- Thymocytes/metabolism
- Zebrafish/genetics
- Zebrafish/immunology*
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/metabolism
- PubMed
- 29066577 Full text @ J. Exp. Med.
Citation
Kasheta, M., Painter, C.A., Moore, F.E., Lobbardi, R., Bryll, A., Freiman, E., Stachura, D., Rogers, A.B., Houvras, Y., Langenau, D.M., Ceol, C.J. (2017) Identification and characterization of T reg-like cells in zebrafish. The Journal of experimental medicine. 214(12):3519-3530.
Abstract
Regulatory T (T reg) cells are a specialized sublineage of T lymphocytes that suppress autoreactive T cells. Functional studies of T reg cells in vitro have defined multiple suppression mechanisms, and studies of T reg-deficient humans and mice have made clear the important role that these cells play in preventing autoimmunity. However, many questions remain about how T reg cells act in vivo. Specifically, it is not clear which suppression mechanisms are most important, where T reg cells act, and how they get there. To begin to address these issues, we sought to identify T reg cells in zebrafish, a model system that provides unparalleled advantages in live-cell imaging and high-throughput genetic analyses. Using a FOXP3 orthologue as a marker, we identified CD4-enriched, mature T lymphocytes with properties of T reg cells. Zebrafish mutant for foxp3a displayed excess T lymphocytes, splenomegaly, and a profound inflammatory phenotype that was suppressed by genetic ablation of lymphocytes. This study identifies T reg-like cells in zebrafish, providing both a model to study the normal functions of these cells in vivo and mutants to explore the consequences of their loss.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping